I am trying to develop

More Simona Stefanovici's questions See All

Does anyone know who developed LAPT broth? What does LAPT stand for?

LAPT broth: 1.5% Bacto Peptone, 1% Bacto Tryptone, 1% yeast extract, 1% glucose and 0.1% Tween 80

07 November 2023 8,166 2 View

Could anyone identify the artefacts in my cell culture?

Hi, I am growing some TK6 cells and have noticed these artefacts in every passage. Is anyone able to identify it as contamination (fungal, bacterial etc) or is it just cell debris/ anything else....

22 August 2023 5,181 2 View

What alarm limits do you set for apnea monitoring your neonates in NICU? What are the clinical reasons for choosing that particular alarm settings?

I have met clinicians setting their inferior limit alarm for apnea, in critically ill children, at 5 seconds; cannot understand this because pauses in ventilation of 5 to 10 seconds are normal...

19 July 2023 4,429 2 View

Surgical Pleth Index limitations, when I can and cannot use SPI tu guide intraoperative opioid administration?

influence of ephedrine on Surgical Pleth Index values during anesthesia. Can SPI still be used to monitor NANB when vasoactive drugs are given?

03 July 2023 1,023 2 View

How does the neighborhood approach uncover power dynamics and promote equitable refugee integration?

How does the neighbourhood approach, which examines local contexts and dynamics, help uncover the role of power dynamics and social hierarchies in shaping the social, economic, and political...

05 June 2023 9,437 0 View

Does anyone have experience with inhibitor remover for removing tert-butylcatechol?

Hello, I have bought a prepacked column of inhibitor remover from Sigma Aldrich. I want to remove tert-butylcatechol inhibitor from styrene and I wonder how much volume of styrene I can use for...

03 January 2023 7,151 1 View

How to permeabilize exosomes to evaluate its cargo through FCM?

Hi everyone, I'm wondering if someone could help me. I want to evaluate the presence of specific proteins in the interior of exosomes but can't find any protocol that would allow us to...

19 June 2022 4,626 0 View

How to permeabilize exosomes to evaluate its cargo through FCM?

Hi everyone, I'm wondering if someone could help me. I want to evaluate the presence of specific proteins in the interior of exosomes but can't find any protocol that would allow us to...

19 June 2022 4,442 0 View

How to permeabilize exosomes to evaluate its cargo through FCM?

Hi everyone, I'm wondering if someone could help me. I want to evaluate the presence of specific proteins in the interior of exosomes but can't find any protocol that would allow us to...

19 June 2022 5,220 2 View

How can I culture NSC34 properly?

Hi everyone, I'm using NSC34 cell line for some experiments. I use DMEM 10% FBS to culture them, but when I seed cells (in a 24-well plate or 96 well-plate) they grow slowly and are poorly...

01 March 2022 2,741 0 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Dhananjay B Meshram

Try looking at solubility and pka of both the drugs. Then you will be able to decide about the pH of mobile phase,

Choon Lee

Glucosamine can be detected using low wavelength (205nm) due to lack of double bond pi electrons on an amino 4.6x150mm column with 5 micrometer packing. pH needs to be higher closer to 7.5 using 3.5g dibasic K2HPO4 buffer 70% aq: 30% acetonitrile. Adjust aqueous to ACN solvent ratio to achieve optimal separation. Good luck, Simona!