I am using a protocol for Bacillus subtilis for which there is a requirement for special electroporation media having sorbitol, mannitol and glycerol. I am using puc18 plasmid (conc 50-100ng/microliter). After heat shock, using 500microliter SOC media and 1 hour incubation, there is no growth observed in Amp plates (conc 100microgm/ml).

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