Will rat colon cancer cells (DHD/K12) derived from BDIX rats grow in Sprague Dawley ?

we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?

23 January 2021 1,437 2 View

How can I indicate to you that I am in fact the author of a book that you have informed me about for quoting other work of mine?

The question concerns this title: Viktor Smith Naming and Framing: Understanding the Power of Words across Disciplines, Domains, and Modalities. Routledge. All the best, Viktor

01 January 2021 4,197 1 View

Who has scheelite (CaWO4) activated by Eu, Sm, Tb, Er or other rare earths?

I would be grateful to my colleagues for the transfer of samples for the purpose of comparison with natural samples in terms of luminescent properties.

03 March 2020 6,099 1 View

Can anyone identify this plant?

This liana grows in the greenhouse of O.V. Fomin Botanical Garden of Taras Shevchenko National University of Kyiv (Ukraine). The plant has been introduced from Botanical Garden in Batumi...

29 January 2020 1,972 16 View

Compatible of QIAseq Stranded Total RNA Lib Kit and Ribominus Transcriptome Isolation Kit?

Dear all, I'm going to perform RNA-Seq with a QIAseq Stranded Total RNA Lib Kit and depletion with Ribominus Transcriptome Isolation Kit/ Ribominus concentration Kit. Does anyone have...

28 October 2019 8,120 1 View

Low bacterial RNA yeld with RNeasy MiniKit?

Dear all, I am trying to do an RNA extraction of different bacterial species with RNAeasy mini kit, protokol #4 (lysozyme lysis + proteinase K digestion). Unfortunately, I have a fairly low...

27 October 2019 5,537 0 View

What is a relation between relaxation time and mean free path for ideal gas?

These two values should be interconnected throufg integral of collisions. But I can not find a clear formula.

02 November 2018 3,613 1 View

Is there a database for bacterial protein mutants?

I'd like to screen on the sequences of available bacterial enzyme mutants (especially P. diminuta PTE enzyme [Uniprot ID: P0A434] ). Is there a database where I can find these mutants collected...

24 April 2018 6,471 2 View

Could you suggest some good algorithms for photo similarity recognition?

I would like to be able to compare photographs of places (mainly indoor) with existing database of photos to geo tag them. If you would be interested, this could lead to some research...

14 September 2017 9,947 3 View

I have L-histidine hydrochloride monohydrate. Help me, please, how can I eliminate HCl and water molecule to obtain L-histidine only?

Will NaOH or KOH neutralize L-histidine hydrochloride? And how can we separate products from each other if it will?

27 June 2017 6,690 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View