08 February 2022 2 5K Report

I labeled E2-BSA conjugates in the past. Unfortunately, the antibodies seem to have non-specific binding to BSA. It would be great if any of you have dealt with such problems.

Thank you,

Parvez

More Parvez Syed's questions See All
What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to simulate 5G Quality of Service characteristics?

For my research, I would like to simulate an end to end 5G network. My goal is to observe the behaviour of SDAP protocol, dynamic configuration of 5QI values etc. To summarize I would like to...

21 February 2021 7,624 3 View

SSR primers are species specific or we can use it for any plant species ?

Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...

20 February 2021 315 4 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

How to get actual or true SoC while predicting SoC for Batteries using Data Driven Approaches?

I have seen it in multiple research articles that authors use certain approaches to predict electric vehicle battery's state of charge(SoC) and then they compare it with battery's true SoC. So I...

11 February 2021 9,070 3 View

How fishery by-products/fish waste can be utilised as efficiently, cost-effectively and eco-friendly?

Fishery by-products utilisation in the industry with minimum cost and low carbon emissions.

29 December 2020 1,752 11 View

Techniques of Machine/Deep Learning for Battery's SoC Estimation?

Hi everyone I want to know which techniques of Machine Learning/Deep Learning I can use for more accurate SOC estimation of Lithium Ion batteries. Thanks and Regards

24 December 2020 2,096 1 View

How can I know which sequence is the correct one if there are lot of predicted sequence in database for my target gene?

Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...

23 December 2020 5,531 12 View

How to rectify the miscomputation of Pressure and Continuity in HVAC CFD Simulation Using OpenFOAM?

I'm using OpenFOAM to run a CFD study of thermal comfort within a hall. I'm using OpenFOAM v1912 and BouyantBoussinesq(Simple&Pimple)Foam solver. eI have 6 inlets and outlets each with specific...

21 December 2020 1,303 3 View

Similar questions and discussions
Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close