I want to quantify the MSG content in several food products. Are there any GC-MS based analysis methods to quantify the MSG content?
Dear Sir. Concerning your issue about the analytical method of mono sodium glutamate content in food products by GC-MS. A rapid, sensitive and simple chromogenic method (Colorimetric Determination) was developed for quantitative determination of monosodium glutamate (MSG) in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H2O2 from a glutamate sample as a result of enzymatic action was measured using 4-aminoantipyrine and phenol as a chromogenic reagent at 502 nm. Glutamate calibration curve was linear up to 125 mmol L-1 with a detection limit of 2 mmol L-1. Or you can use the rapid simultaneous determination of monosodium glutamate (MSG), benzoic acid (BA), and sorbic acid (SA) in canned food and other processed food samples by a method that combines in-capillary derivatization with separation by capillary electrophoresis. This method employs the rapid derivatization of MSG with o-phthalaldehyde (OPA) in the presence of 3-mercaptopropionic acid (3-MPA) and enables the detection of the resulting OPA-MSG derivative and of non-derivatized BA and SA at 230 nm . Also you analyzed by a simple, fast, specific, and precise high performance thin layer chromatography method which has been developed for the estimation of monosodium l-glutamate (MSG) in food products. Aluminum plates precoated with silica gel 60 GF254 were used as stationary phase and a mixture of methanol–chloroform–formic acid in the ratio 5:5:1 (v/v) as mobile phase. Quantification was carried out by post chromatographic derivatization using 1% ninhydrin solution, and the developed spots were scanned by using a densitometer in absorbance mode at 485 nM. The Rf value of MSG was 0.64. The results of the analysis have been validated statistically and by the recovery studies. Linearity was observed in the concentration range of 400–1000 nG. I think the following below links may help you in your analysis:
http://www.cibj.com/Upload/PaperUpLoad/477b57a9-5aee-411b-ad7a-ff70a5c5e0b0.pdf
http://www.sciencedirect.com/science/journal/00219673/1449
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964770/
Thanks
MSG is Monosodium Glutamate which dissociates. Therefore, you can try an enzyme kit. See attached;
https://secure.megazyme.com/L-Glutamic-Acid-Assay-Kit
I am working on the MSG analysis in spice with LC/MS. When I tuned the MSG solution, I found only glutamic acid (MW 147) see m/z of 148 in pos. I don't know if this is normal when MSG is in a solution, Na dissociates out of the molecule.
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