Hello:
Does anybody have experience culturing ciliates grazing on cyanobacteria? I have an environmental sample with Nassula grazing on Microcoleus and would like to keep the ciliate living in lab culture. I would love to talk with somebody about ciliate's culturing conditions, food requirements, starvation, and so on. Thank you in advance for your help!
I have experience culturing ciliates paramecium bursaria with chlorella. They live well on Lozin-Lozinsky's medium with yeast (sarcomyces).
Hello, Rosalina, how are you? I'm not sure how to cultivate just a single species, but a cultivation technique that has worked well in our laboratory is to prepare a bacterial culture of raw rice with tap water... just break up a few pieces of rice grain, add to a falcon tube, put about 10mL of water in the tube, close it and wait about 2 days for there to be a considerable amount of bacteria, then just use that water in isolated cultures of protists to feed them whenever you need to. I hope to help a little with my comment, hugs!
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