QPCR contamination - how to solve my problem?

I am having a terrible time with qPCR contamination on fish genes. I have ordered new primers, only used filter tips, sprayed my pipettes and work area with 10% bleach , tried to complete inside a...

18 September 2018 4,572 3 View

How do you decide what species to include in phylogenetic tree for paml positive selection analysis?

Hi, I am trying to test if one copy of a duplicated gene is under positive selection in 1 particular fish species. The other copy of the duplicated gene has been observed to be under positive...

04 September 2018 1,489 1 View

I have been performing simultaneous TGA/DSC runs on a polymer. I am getting a 60% mass loss but no exo/endothermic reaction to accompany. Any ideas?

The reaction takes place routinely at 550C with no change in energy. Has anyone experienced this before?

18 December 2017 4,207 13 View

Can I reuse primary and secondary antibodies for immunohistochemistry?

In a lab I rotated in, we always reused primary antibody but never reused secondary. In the lab I've chosen to remain in, it is considered okay to reuse secondary antibodies. I am a bit hesitant...

27 September 2017 3,822 7 View

Can both definitions of the native conformation of a polypeptide/protein be reconciled?

01 February 2016 9,299 2 View

Can intact microbial cells have antioxidant activity?

I have currently working on probiotic yeasts. Can an intact cell have antioxidant activity without lysing to get the intracellular content?

01 January 1970 8,842 3 View

Literature on Knowledge Management in Educational Institutions

Hi all, I'm currently working on my dissertation and I'm looking for more leads on different journals, books, websites on knowledge management in educational institutions. Your help will be...

01 January 1970 4,599 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View