Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Is it possible to full-couple the interFoam solver with the chtMultiRegionFoam solver?

We need to calculate multiphase stream of the one substance in the air atmosphere (this is why we using interFoam), but also we need to get heat transfer data between the substance, solid obstacle...

11 January 2021 872 2 View

Does anyone have experience with Land use/land cover data extraction from Second military survey maps?

I am trying to create polygon shapefile with land use from Second military survey using Arcmap. However, I really have hard time with reading the map, more correctly with boundaries between...

07 November 2020 6,018 3 View

How do I remove Bacillus sp. spores from Kraft Paper machine?

In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...

03 November 2020 1,726 10 View

What volume of a cell lysis buffer does one use for cells?

I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...

03 November 2020 6,330 3 View

Best protocol to perform Western Blot from mice brain?

I am trying to look at the expression of c-fos nuclear protein in mice brain using WB technique. Can anyone suggest a protocol to extract the proteins? Better harsh homogenate before sonication?...

27 October 2020 5,272 2 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

25 October 2020 8,600 1 View

What do you think now of virtual teaching compared to face-to-face teaching of finished transmission of knowledge?

21 September 2020 4,178 10 View

How to store W/O droplets for over 10 days using paraffin oil with Span 80?

I am currently using a PDMS chip to produce water droplets with a diameter of 200 um in paraffin oil with 3wt%. After a couple of hours, the stationary droplets stored in the chip starts to form...

18 August 2020 2,459 3 View

How do you use factor analysis on categorical variables?

Hi, I'm trying to identify how similar different individuals are in their body traits. Normally, I would use a PCA or DFA, depending on my question. However, I have only categorical variables...

20 July 2020 4,171 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View