How to identify and retrieve many (+30) RNASeq datasets (Fasta) under a specific criteria in a practical way?

22 March 2020 3,649 3 View

What have been the contributions of cultural studies to the teaching of English as a second or foreign language?

the idea that intercultural competence develops communicative competences. Worldwide, this relation intercultural-communicative competence has been studied by teachers and researchers (Agudelo,...

01 February 2020 9,056 3 View

How to conduct a Chi-square analysis dealing with two independent groups?

Hello fellow scholars, I would like assistance with a Chi-square analysis dealing with two independent groups. In one research article I was reading, the researcher was studying US attitudes...

25 January 2020 7,039 6 View

How is the Linguistic Colonialism evidenced in the English Textbooks of Multinational Publishing houses in teaching of English as a second language?

The purpose of this question is to examine and compare the concepts of linguistic colonialism and cultural alienation in University textbooks for teaching English as opposed to the theories about...

01 January 2020 1,603 3 View

How to connect beam188 and link180 elements in ANSYS APDL?

I am currently working on the design of a deployable truss structure and I would like to know what is the proper way to connect beam188 and link180 elements in APDL? The diagonals (purple lines)...

07 April 2019 3,152 4 View

What's the amplified gene in the DNA ladder assay for apoptosis detection?

Greettings, I need some guidance. Regarding to the DNA ladder assay for apoptosis detection which is based on electrophoresis analysis, I would like to know what is the gene to be amplified in...

03 April 2019 9,559 4 View

Protein misfolding behaviour?

Hi all, I am working on a tricky protein. I surpassed some initial aggregation behaviour by adding 0.3% CHAPS and 1mM TCEP, buffer in which is happy until the removal of the SUMO tag, then it...

09 December 2018 9,460 3 View

In a Real Time PCR, is a CT of 4.6 (average) enough to determine presence of babesia/anaplasma in a sample?

We are currently trying to identify presence of babesia bovis, babesia bigemina, and anaplasma marginale in DNA isolated from white blood cells. However, the amplification plot values start...

12 November 2018 4,051 3 View

Hi everyone, someone have made real time PCR in tissue fixed ?

for us is very importan the purifiaction and extration of the mRNA in tissue previusly fixed with paraformaldheide, thats possible? or may be we can used other solutions for preserve the sample,...

04 May 2018 8,912 0 View

Does anyone know a clustering algorithm/software that transforms a group of images into an undirected weighted graph?

Hi everyone. I'm currently trying to assess the ability of human volunteers to cluster a set of images into a fixed number of clusters according to perceived visual similarity (the images are...

14 January 2018 8,222 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View