11 Questions 123 Answers 0 Followers
Questions related from Luisa F. Jimenez-Soto
I am in need of the knowledge of a biophysicist with speciallity in membranes and/or lipids. Please let me know if you will be interested in exchange of knowledge and a cool project.
09 September 2014 6,569 4 View
I was thinking of fixing tissues/cells with osmium tetraoxide, since it has been described to reduce the autofluorescence of tissue preparations. However, I was strongly discouraged by the...
11 November 2013 6,762 8 View
We are standardizing a method to Hybridize DNA from Colonies onto Nylon membranes. We already have two protocols to try, but what I would like to see at the end of the "try outs" is if the DNA: 1)...
06 June 2013 5,923 2 View
I have been testing a NFkB luciferase reporter. I have always done it in HEK293 cells and the positive and negative controls are working fine. But as I changed to epithelial carcinoma cells, the...
11 November 2012 1,343 2 View
Dear all, after creating several mutants containing a myc-tag, I have discovered that in infection conditions, my anti-myc antibodies are not able to recognize the tag but they do recognize it in...
08 August 2012 4,026 9 View
Hi! I am doing some microinjection of cells, but sometimes, they get more damaged than usual. My question is: For the fast reparing of membrane integrity after permeabilization experiments, a...
08 August 2011 2,740 1 View
Dear all, I have been trying to run my samples (cellular lysates) on an SDS-agarose gel (low melting). The first try outs were terrible, but I gain some knowledge about my limitations (like...
06 June 2011 8,309 15 View
Dear All, I know that many write here messages in order to get help with parts of the protocols that seem not to work. Since many of us with a little bit more of experience want to help, I would...
03 March 2011 6,331 21 View
Hi! I am looking for a research group in the area of Munich that has access to a transmision electron microscope and that will be willing to show me how to work with it, so I can confirm my...
12 December 2010 5,222 3 View
Hello Everyone, I am trying to reproduce the EXACT conditions (at least as close as possible!) for the cytosol in my solutions. From Ca+2 I have found the physiolocal conditions between...
01 January 2010 6,158 1 View
Dear all, I have a question for the community. I sent a manuscript to a well known journal. After asking about the reason for a very long delay in the process, the Journal informed me that the...
01 January 1970 9,261 7 View
