I am getting RNA 18s band thicker or similar to 28s band any explanation for this, can i consider my RNA intact ?

Ghalia Aboualchamat @Ghalia-Aboualchamat

23 June 2018 1 7K Report

i am working on DNA methylation level of cancer-related genes and their expressions in colorectal cancer.i am using trizol for RNA extraction but i usually get 18s band thicker than 28s or similar. any explanation for this result?

thank you

Craig Silver

per:

https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rna-isolation/tech-notes/is-your-rna-intact.html

If you have a much larger 18S band than 28S, its because your 28S RNAs degraded. The ratio of 18 to 28S RNA is often indicative of RNA quality.

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