I am getting almost same pattern of expression of different gene in real time PCR?

More Vijayata Singh's questions See All

Can anyone just me the methodology to get oral secretions from Trichoplusia ni. (cabbage looper) caterpillars?

how to get pure Oral secretions from the third instar larva of cabbage looper caterpillars? Is there any easy method?

06 July 2017 625 0 View

Should we take care of genotypes positional arrangement while performing herbivory experiment in petridishs?

I am doing herbivory experiments with T.ni caterpillars. I do have some susceptible and resistance plants variety. While arranging them in petriplate I think there should be some tricks. I mean...

05 June 2017 3,042 21 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Praveen K Singh

Hi Vijayata, Have you checked for gDNA contamination and also are you talking about relative or absolute expression? It is very important to check for relative expression and also there should be almost no genomic DNA contamination in your samples.

Vijayata Singh

There was no gDNA contamination in the samples. Melting curves are fine. here, i am talking about relative quantification.