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Questions related from Praveen K Singh
Can anyone help me with joining two pcr fragments using primers extensions rather than using ligation protocol?
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I am digesting PCR products with XbaI, KpnI ( 1st pcr), XbaI, BamHI ( 2nd pcr) and then my vector with BamHI and KpnI. After purifying my vector and all PCR, I put all nucleic acid fragments...
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