I am determining Ferrous and Ferric iron species(Fe-2 and Fe-3) by Colorimetric method termed 1,10-Phenanthroline. I am getting the higher absorbance(0.100-0.500) for total iron standards and even samples than the Ferrous ion standards and samples which absorbance is less (0.020- 0.040). But when I put the absorbance in regression equation, the reading of Ferrous iron in comparison of Total Iron goes on higher side which absorbance was less than Total Iron. I have performed the analysis and calculation many times but unable to resolve this issue. If some scientist has the expertise of this method, He may please guide me as my research is stuck for months. Thanks
you need to dilute the sample so that their absorbance values fall with those of the standard solution.
If the abs. is high then the conc. should be high
You have got 0.1-0.5 abs. for total and 0.02-0.04 abs. for ferrous then the conc you will get for ferrous is less
The problem seems to be in your regression if i understand your question
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