Weak SSR peak on capillary electrophoresis trace - is it a heterozygote?

Looking at the attached image, the top two samples show a weak peak at 212 compared to the very strong 200 peak. The bottom samples is homozygous for the 212 peak. Since the samples look a bit...

27 October 2020 2,353 3 View

How the outer voltage loop generates grid current reference for an inverter?

Hello, I am working on grid connected single stage PV system. I am going with SRF control (dq-control). what I found more complicated is outer voltage control loop. In outer control loop, Vref (pv...

04 September 2019 3,287 2 View

Can you do immunofluorescence on slides that have already been stained by Diff-Quik?

Slides of BALF were stained with Diff-Quik. Can we stain the same slides for immunofluorescence?

05 September 2018 8,266 1 View

PBMC-PHA assay media yellow

Hi there, just wondering if anyone has the same issue, and if there is something you do, or just something that is accepted. I am performing a PBMC-PHA stimulated assay in 96 well plates. this is...

20 July 2016 7,968 2 View

Concentrating phosphoprotein samples using a centrifuge concentrator?

I'd like to further concentrate a set of cell lysates and then be able to perform a western blot on these samples to look for phosphorylated Vs non phosphorylated protein levels. I am harvesting...

03 March 2015 8,114 12 View

Can we apply concrete damaged plasticity model in abaqus for Rigid Polyurethne foam? And how do I find the parameters for the same?

25 September 2014 3,332 4 View

Duplicate Luminescence Readings

Hello, I am doing a cytotoxicity test. I am using the CellTiter Glo 2.0 Assay, to measure ATP with Luminescence. I use the Cytation 5 to do my luminescence readings. I have a lot of variability...

01 January 1970 3,792 3 View

ATP Assay strange results

Hello, I recently did an ATP assay to detect cell viability. I used the following ATP Determination kit - https://www.thermofisher.com/order/catalog/product/A22066#/A22066. Does anyone know the...

01 January 1970 5,905 3 View

Cell culture media + treatment solution

01 January 1970 8,388 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View