i am following protocol is ---
root tip was fixed in fixative for 3 hour at 4-6 degree Celsius then hydrolysed with 1N HCl for 15 min and finally stain with acetocarmine for 2 to 5 min and also try for 24 hr.
where i was doing wrong? pls guide me
Dear colleague,
You can find usefull informations in the following books:
1. R. Martoja & M. Martoja, 1967. Techniques D’histologie Animale, Masson et Cie Ed, Paris,
2. M. Gabe, 1976. Histological techniques. Springer Ed. Paris.
Best ergards N. Soltani
respected sir, i was not getting result in plant root cell. can you suggest me reference regarding only plant root cell.
Sorry I have worked only on animal models !
Best regards N. Soltani
Your fixative determines whether your colouration works or not. Recommended is Carnoy's fixative or no fixation at all. Staining up to 2-7 days is possible for whole roots.
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