Shilpa Varma

5 Questions 8 Answers 0 Followers

Questions related from Shilpa Varma

If organic fertilizers have low pH than what we should add in to maintain pH in 6-7 range?

we are in process of making organic fertilizer to improve crop production. we use different things but has difficulty to maintain pH. we want to add something which completely natural and increase...

07 July 2019 9,832 26 View

For TLC chromatography how we select solvent system via hit and trial method?

i have work on pure compound isolation of different phyto-chemicals, i need different solvent system for hit and trial methods?

08 August 2016 3,446 9 View

I am doing micro nucleus assay by using acetocarmine, but still i am not getting any result. what should i do?

i am following protocol is --- root tip was fixed in fixative for 3 hour at 4-6 degree Celsius then hydrolysed with 1N HCl for 15 min and finally stain with acetocarmine for 2 to 5 min and also...

02 February 2016 9,298 4 View

Why is HNO3 used in sample digestion process for icp-ms or aas; can we use some other acid such as HCl or H2SO4?

i want to estimate the metal present in our plant sample, for this initial step is sample digestion. many reference paper use nitric acid, is there any other method which can follow. need protocol...

10 October 2015 10,191 13 View

What is a best method for finding the phytochelatins or metallothioneins (MTS)?

phytochelatins is metal peptide bonds occur due to heavy metal stress, or it is a defence mechanism of plants. my queries is for best possible lab method to determine this pc bond in plant? i need...

08 August 2015 10,111 3 View