Hi Komal,

You may approach the problem this way:

Your stock antibody concentration is 0.42 mg/ml or 420 microg/ml

Final concentration you need is 0.5 microg/ml

Final volume is 100 microL

So, the volume of stock antibody concentration would be

100 microL X 0.5 microG/ml divided by 420 microG/ml,

which is equal to 0.12 microL

Basically, this follows the simple equation of N1V1 = N2V2

Hope this helps,

Debabani

HI Debabani,

Thanks, i tried