Komal k kumar j

9 Questions 25 Answers 0 Followers

Questions related from Komal k kumar j

I am confusing with antibody dilutions can any one help me ?

I have purified an antibody the final concentration is 0.42mg/ml. i want to titrate antibody, so that i can pick up 0.5 microgram/ml to 5microgram/ml concentration in 100 microL staining medium. i...

01 January 2015 2,632 2 View

Can anyone tell me how to save Human Leukemic cells after treating with drugs before preparing peptides for Mass spectra?

Can anyone tell me how to save the cells (Human Leukemic cell lines) after treating with drugs before preparing proteins for Mass spectra? i have other things to do so i have planned to save the...

12 December 2014 6,334 2 View

Can anyone tell me the suitable stimulation conditions to stimulate pStat1, pStat3, pStat4, p38 and pAkt?

Hi guys. Can anyone tell me the suitable stimulation conditions to stimulate pStat1, pStat3, pStat4, p38 and pAkt. I am working with mouse spleen, bone marrow and murine cell line BaF3. I am...

11 November 2014 8,178 2 View

Can anybody help with multicolour flow cytometry analysis?

I performed multicolour analysis in flow cytometry. I stained for FITC, PercpCy5, PercpCy7, PE and PB on cancer cells. I compensated using Beads and used FMos for gating population, but when I run...

04 April 2014 4,129 14 View

Phosphoflow: I am working on cancer cell lines looking for phosphoproteins by flow cytometry. Any advice?

In flow cytometry single histogram of a single phosphoprotein splitted into two peaks. Even that is a stable cancer cell line. Why is this?

12 December 2013 631 0 View

Why does a single histogram sometimes split into two peaks for a single phosphoprotein in flow cytometry?

I am working on phosphoflow for specific proteins. In the histogram sometimes a single histogram split into two peaks for single phosphoprotein, but sometime for the same protein that peak...

12 December 2013 5,669 5 View

Green fluorescent proteins peak is too high at FITC channel for all the samples - any suggestions?

I added PI to the cell line that I am interested to exclude dead cells. Then run the experiment in FACS canto. But GFP is sowing the peak at 10 to the power of 5 in FITC channel for all the...

12 December 2013 5,455 5 View

Any advice on checking for green fluorescent protein in one of the cancer lines in FACS canto?

Which channel should I use to see the population of GFP? Can I use the APC channel?

12 December 2013 9,186 9 View

What should nonphosphorylated and phosphorylated proteins looks like in in the histogram?

I am working on phosphoflow in FACS and have stained the cells with surface markers and intercellular markers. I got the FSC and SCC results and histogram but I am confused about how to interpret...

11 November 2013 10,019 4 View