I work with infarcted brain tissue and I am checking the MMPs expression. The thing is that the gels are kind of dirty (specially around the top of the gel), and I would really appreciate if someone could give me any advice!! Is this normal with brain tissue? Gels are running for 2 hours and a half at 120V, then they are "cleaned" in a solution containing Tritón and incubated for 40 hours at 37º.
Thanks!!
Some of the gelatin has electrophoresed out of the top of the gel. Try a lower running voltage. If you cast your own substrate gels try a different bloom number gelatin. There is not a strict correlation of bloom number with molecular weight, but in general a higher bloom number is better.
I agree that your bloom number is fine. Also, run the gel in a coldroom, refrigerator or a gel rig with a cooling system.
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