Is it normal after a co-culture values to mono-culture are almost similar?

I performed a co-culture using Kluyveromyces marxianus and Pichia stipitis and I obtained an ethanol concentration of 11.5 g/L and for the monocultures were 10.6, and 8 g/L respectfully. When I...

04 May 2018 235 3 View

Can anyone give me advice on my protocol for yeast co-culture?

Basically, I am working on two yeast strains: Kluyveromyces marxianus K-21 and Pichia stipitis 21777. K-21 is a faster growing yeast as compared to BCRC 21777, my procedure is as...

04 May 2018 6,282 2 View

Could anyone suggest me a simple procedure for cellulase re-usability?

I am working on an experiment to produce bioethanol from Ulva lactuca. In the pretreatment process I am using 5 g in 100 ml 0.4 N HCl at 121oC for 20 mins. After this procedure I add about 4560 U...

02 March 2018 7,263 2 View

Has anyone ever used cellulase in its powdered form?

I am working on an experiment using cellulase ap3 for enzymatic hydrolysis. In my previous experiment I directly added the powder to my acid hydrolysate at a pH of 4.5. However I am not sure it...

11 December 2017 9,362 3 View

Can anyone help me with a complete and simple design for hydrolysis of seaweed?

Right now i am planing to perform a hydrolysis on seaweed. The problem I have is that I am inexperienced and have never done it before. So first I am planning to take 5 grams of the seaweed and...

11 December 2017 8,583 3 View

Is just filtering L-Rhamnose monohydrate ; its reagent grade in 0.22 ul enough to make HPLC grade?

07 August 2017 5,400 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Western blot transfer problem - what could cause it?

Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...

25 January 2021 4,228 6 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View