Dan Med

7 Questions 4 Answers 0 Followers

Questions related from Dan Med

Is it normal after a co-culture values to mono-culture are almost similar?

I performed a co-culture using Kluyveromyces marxianus and Pichia stipitis and I obtained an ethanol concentration of 11.5 g/L and for the monocultures were 10.6, and 8 g/L respectfully. When I...

05 May 2018 267 3 View

Can anyone give me advice on my protocol for yeast co-culture?

Basically, I am working on two yeast strains: Kluyveromyces marxianus K-21 and Pichia stipitis 21777. K-21 is a faster growing yeast as compared to BCRC 21777, my procedure is as...

05 May 2018 6,315 2 View

Could anyone suggest me a simple procedure for cellulase re-usability?

I am working on an experiment to produce bioethanol from Ulva lactuca. In the pretreatment process I am using 5 g in 100 ml 0.4 N HCl at 121oC for 20 mins. After this procedure I add about 4560 U...

03 March 2018 7,298 2 View

Can anyone help me with a complete and simple design for hydrolysis of seaweed?

Right now i am planing to perform a hydrolysis on seaweed. The problem I have is that I am inexperienced and have never done it before. So first I am planning to take 5 grams of the seaweed and...

12 December 2017 8,614 3 View

Has anyone ever used cellulase in its powdered form?

I am working on an experiment using cellulase ap3 for enzymatic hydrolysis. In my previous experiment I directly added the powder to my acid hydrolysate at a pH of 4.5. However I am not sure it...

12 December 2017 9,391 3 View

Is just filtering L-Rhamnose monohydrate ; its reagent grade in 0.22 ul enough to make HPLC grade?

the sample I got was L-Rhamnose monohydrate but its reagent grade. Is just filtering enough to make it HPLC grade? Or are there other methods. I am going to use the rhamnose in order to make a...

08 August 2017 5,433 5 View

I am about to do immobilization of yeast to PCS for bioethanol production using seaweed. I am wondering if there may be a standard procedure?

I will be using the yeast Kluyveromyces marxianus. Basically the procedure I was taught was to place to the PCS (weigh it first) into the broth containing the yeast (YM medium) and leave there for...

06 June 2017 5,427 4 View