Is it normal after a co-culture values to mono-culture are almost similar?

I performed a co-culture using Kluyveromyces marxianus and Pichia stipitis and I obtained an ethanol concentration of 11.5 g/L and for the monocultures were 10.6, and 8 g/L respectfully. When I...

04 May 2018 235 3 View

Can anyone give me advice on my protocol for yeast co-culture?

Basically, I am working on two yeast strains: Kluyveromyces marxianus K-21 and Pichia stipitis 21777. K-21 is a faster growing yeast as compared to BCRC 21777, my procedure is as...

04 May 2018 6,282 2 View

Could anyone suggest me a simple procedure for cellulase re-usability?

I am working on an experiment to produce bioethanol from Ulva lactuca. In the pretreatment process I am using 5 g in 100 ml 0.4 N HCl at 121oC for 20 mins. After this procedure I add about 4560 U...

02 March 2018 7,263 2 View

Can anyone help me with a complete and simple design for hydrolysis of seaweed?

Right now i am planing to perform a hydrolysis on seaweed. The problem I have is that I am inexperienced and have never done it before. So first I am planning to take 5 grams of the seaweed and...

11 December 2017 8,583 3 View

Is just filtering L-Rhamnose monohydrate ; its reagent grade in 0.22 ul enough to make HPLC grade?

07 August 2017 5,400 5 View

I am about to do immobilization of yeast to PCS for bioethanol production using seaweed. I am wondering if there may be a standard procedure?

05 June 2017 5,372 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View