I'm using hyaluronidase (HA) to isolate cells from synovial fluid. I use a concentration of 200 U per millilitre of synovial fluid. For particularly viscous samples, I have read that this can be increased to 400U.

After 30 min incubation at 37oC, I centrifuge the samples at 1000 xg for 10 min. If the synovial fluid remains cellular, I dilute 1:2 with PBS-EDTA and spin again.

My questions:

1. Are there any known negative effects on cell viability/function when using HA?

2. Other researchers want to put the synovial fluid through the mass spectrometer. Are there considerations to be taken into account given that HA has been used on it?

Many thanks for your answers.