Hyaluronic acid modified with EDC/NHS?

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Karthik Raman

Take a look at the attached method on SciGine related to covalent bonding of proteins to hyaluronic acid for SPR. They use EDC/NHS do their coupling.

http://scigine.com/method.php?ID=170701

Farah Nurlidar

The carboxy group of HA can be activated using EDC/NHS reaction.

You can read this paper: http://pubs.acs.org/doi/pdf/10.1021/bc00010a007 (Chemical Modification of Hyaluronic Acid by Carbodiimides)

or if you want to know the principle of the reaction using carbodiimide, I recommend: Book: Bioconjugate Techniques (part 3, Zero-length cross-linkers) by Greg T Hermanson.