Human pancreatic islet dissociation?

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Could I use a plasma/serum insulin ELISA assay to dose intracellular insulin content?

31 December 2015 5,442 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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The Curse of Evolution and Complexity?

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I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

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How to retain the a GFP tagged gene expression in stable cell line?

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Could you try using PeptiCloud and see if it's a useful tool for biology research?

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Abbas Sabbar Dakhil

1)Assessing islet cellular composition and beta cell viability using Flow Cytometry (FC) and Laser Scanning Cytometry (LSC) .

2)staining with ductal marker CA19, with Newport Green (NG) and FluoZin3 (FL3) for beta-cell identification

Ammar Mohammed

hi Nicolas.

did you try immunohistochemistry protocol ??

Nicola Marrano

Hi Ammar

no, I've not tried it. Do yuo think it could be bettere?

yes I think so.

it was my project few years ago but I couldn't proceed it for some personal reasons.