7 Questions 11 Answers 0 Followers
Questions related from Nicola Marrano
I need to understand if an antibody I'm using is specific or not. The antibody is not validated. In immunoblotting, the antibody detects a prominent band at the right molecular weight and two...
04 April 2019 5,518 5 View
Dear all, I have paraffin embedded brains from C57BL/6 mice. I usually extract mRNA from slices of 20 micrometer, randomly sliced from the entire organ. Is there a way to identify the hypothalamus...
10 October 2017 6,342 3 View
I want to use a neutralising antibody to neutrilize a circulating hormon in mice, but I have not experience. 1. Do I have to use necessarily a mouse antibody? 2. The experiment lasts 14 days....
09 September 2017 2,170 3 View
I would like to see beta cell apoptosis in human pancreatic dissociated cells. I thought I would make a costaining: TUNEL to detect apoptosis and Insulin to mark beta cell. Someone has ever done?...
02 February 2017 5,256 4 View
Do you have any protocol for human pancreatic islet cells dissociation? Thanks
02 February 2017 1,356 4 View
I'm performing a real-timePCR experiment (Applied Biosystem step one plus) using SyberG as fluorescent dye and ROX as passive dye. At the end of the run, only in some wells, the software gives me...
03 March 2016 5,027 4 View
Could I use a plasma/serum insulin ELISA assay to dose intracellular insulin content?
01 January 2016 5,415 4 View
