Is there a way I can differentiate peripheral human memory B-cells into Ig secreting cells (ISC) via T-independent pathway? I understand in vivo this can happen through LPS stimulation in presence of BAFF/APRIL. But I wanna know if we can do this ex vivo using humen memory B-cells from PBMCS.
Thanks :)
you can also stimulate B cell with TLR9 ligand. CpG is better than LPS in inducing Ig secreting cells.
Hi Shubhranshu,
I am trying differentiating Human Memory B cells in to Plasma cells with R848 and IL2 but not getting enough differentiation with these stimulator. Have you been able to differentiate Human Memory B Cells in to plasma cells in your experiment? Did you use CpG2006?
Yes, I was able to differentiate them. I used a cocktail if CpG2006, IL2, IL15, IL10 and BAFF.
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