Hello,

I am trying to differentiate HT-29 cell line using Methotrexate (MTX). I came across an old article that suggested treating cells for 3 months in order to reach their confluence state, leading for mucus secretion.

I was wondering if anyone here could suggest a protocol for differentiating the cells with MTX, so they could be more morphologically similar to goblet cells and secret mucus.

Thank you

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