How to cite a reference for a literature review?

Someone confuse me, he said that when you have some information you want to use it from reference say X, and the reference X inspired this information from reference say Y, then so, I should cite...

21 September 2020 8,630 3 View

Would it be possible to analyze plant RNA-seq data in order to capture any transcripts from other organisms (e.g. Viruses, Bacteria, etc.)?

We have RNA-seq data of different plant tissues; Is it possible to analyze data in order to capture any transcripts from other organisms (e.g. viruses, bacteria, etc.) ?

01 July 2019 5,143 4 View

Is the PVP polymer soluble in 0.1 HCl ??

I want to use 0.1 HCl as a solvent to identify the drug content of a PVP-based formulation.. could I ?

20 April 2019 6,273 3 View

Three-dimensional Gel Electrophoresis?

Does anybody have information regarding using 3D electrophoresis in the studying gene expression? Does this method could analyse the expression of all genes in the organism or it target specific...

27 November 2018 8,071 3 View

Does anybody have an idea regarding minus value in statistic analysis?

18 May 2017 5,383 10 View

Does anybody comfirmed the TOXA:GFP transformants with southern blot?

I transformed my strain with TOXA:GFP and trying to confirm the insertion by using southern blot technique. I can see the fluorescent under the microscope but it does not work with ELISA or...

27 April 2016 2,870 2 View

What is the difference between using southern blot and gene complementation after a knockout gene?

Why did we use complementation inspite of how we examine how many copies of the gene mutated by southern blot?

03 May 2014 828 1 View

When I developed my southern blot, I found a black dot that covered and hid my results. What should I do to solve this problem?

DIG southern blot

07 April 2014 1,388 12 View

When selecting suitable enzyme for Southern Blot, should it be cut on ORF, LF, RF in wild type and hygromycin, RF, LF in mutant?

I am confused about selecting of enzyme for a Southern Blot and where exactly to cut it. I am working with knock out and used hygromycin selectable marker.

26 February 2014 510 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View