Priyabrata Halder

5 Questions 6 Answers 0 Followers

Questions related from Priyabrata Halder

Why my patch pipette resistance varies so much with different internal solutions?

I mostly do voltage clamp recording using caesium methanesulfonate based low Cl- internal solution. I use a Narishige vertical puller to pull my electrodes. I constantly get a pipette resistance...

02 March 2023 2,768 8 View

What is this rapid event in patch clamp recording?

I am trying to record glutamate and GABA responses with ChR2 stimulation from striatal MSNs, and I am finding this weird spike in some of my recordings, but not in most. I am using a low-Cl...

16 November 2022 7,268 1 View

Why Clampex is showing pA for both primary and secondary inputs in both recording modes?

I have set everything up according to the guide provided by Molecular devices, and according to them, if the lab bench and the telegraphed instruments are set properly, clampex should...

30 September 2021 1,420 5 View

Why do fluorophores show higher excitation only at specific wavelengths? Why not at all lower wavelengths (high frequencies)?

Why fluorophores show this typical excitation curve? Why not show same level of absorption at all wavelengths below the indicated level? I understand they show some absorption at higher frequency,...

14 July 2016 3,267 2 View

What is the exact equation for V[surs] calculation for CE in Cavalieri estimator?

In the equation of CE calculation, I've got a little confused with the one to calculate V[surs]. It's something like V[surs]= a[i]*(3.(A-V[noise])-4B+C). Everything else is clear to me, but what...

12 June 2014 3,754 2 View