Hi,
I may be able to help you here.
I still have a lot of reagents for the PapType13 test (Jenkins A, Allum AG, Strand L and Aakre RK. Simultaneous detection, typing and quantitation of oncogenic human papillomavirus by multiplex consensus realtime PCR. J Virol Methods, 187: 345-351 (2013)) left over. We never managed to find an investor to bring the test to market and the company folded, but it's a good test and we'd like to see it used, even though we don't have any commercial interest in it any more.
Whether it is suitable for uses would depend on the instrumentation you have available. You need a AB7100, 7300, StepOne Plus, or another instrument that can detect FAM, VIC, NED and ROX simultaneously.
DNA extraction is not part of the kit, so I can't help you there, though I could probably come up with some suggestions that won't cost too much. One of the QIAGEN genomic DNA kits would probably work well. Or you could just do an old-fashioned proteinase K - SDS - Phenol/Chloroform extraction.
At this stage, I have almost completed a clinical sample (Cervical swabs) collection process. Initially, when I planned my project, the price of the kits have been fair and I thought that the extraction and detection kits will easily be available on the market. But, now, I faced solid challenges to get the Extraction and Detection kits from the market mainly due to the inflated prices of the kits.
Is there anyone who can suggest to me how can I contact individuals or laboratories or companies to donate/ help me and allow me to conduct the molecular tests (genotyping) on the collected clinical samples, please?
Dear Dr. Andrew Jenkins thank you very much for your kind support and prompt reply. Privately, I want to share the condensed forms of our test protocol with you. So, can I get your e-mail address, please?
Thank you!
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