I would like to assess the molecular weight, or even fractionate glycoprotein. I have a few question for this assay.
1. Is purification necessary ? if my sample may contain peptide, glycopeptide, glycoprotein etc.
2. I read some paper using HPGPC for glycoprotein MW assessment. what is the difference between HPLC and GPC and HPGPC ? is it a different instrument?
2. Can i use a GPC/SEC column in HPLC system to get all these info? i.e. MW, separation etc.
if i were to use similar parameters/condition in GPC in HPLC system with a GPC/SEC column, does that make it a HPGPC?
Kindly check https://www.shimadzu.com/an/service-support/technical-support/analysis-basics/basic/55/55intro.html
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556795/
Each identifying techniques has an own pros and cons. According to the aim of study, the existing techniques are selected that it is usually could be selected and tested (trial).Then, the made results are compared together in terms of quality and quantity. Finally, the best method for target experiment would be selected.
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