Dear all,
I am growing genetically modified S. typhimurium SL1344 cells in a chemically defined medium in a bioreactor. The cells have a deletion in the aroA gene, rendering them auxotrophic for aromatic amino acids, PABA (para-Aminobenzoic acid) and DHBA (dihydroxy-Benzoic acid). Additionally the strain SL1344 is Histidine auxotroph.
For a better understanding of the consumption of these chemicals and to better control the whole system, I would like to be able to measure the concentration of each additive by HPLC. Ideally in a way it can be automated in a later stage.
Since i have not a lot of experience with HPLC (basic knowledge) and i am struggling to find good resources, I would be happy about every input, advice or reference recommendation.
Cheers!
Hi Simon,
maybe this link will be helpful to you:
https://securecell.ch/wp-content/uploads/2019/09/AN_004_NumeraAAA.pdf
Regards
Markus
Hi Simon, just wabted to share our article if you find it usefuk. Good luck!
https://link.springer.com/article/10.1007%2Fs13361-018-2093-9
Dear Simon,
this task is not to easy. Common protocols for amino acid analysis by HPLC includes derivatization of the amines. Keep this in mind for analysis of benzoic acid.
Best,
Simon
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