What statistical analysis would I need for a questionnaire?

Let me first start by saying I'm dyslexic, two universities were unable to teach me research methods and statistics in a way that worked with my dyslexic limitations, and I've continuingly failed...

08 July 2020 8,712 6 View

I'm looking funding and grants for a medical study in deep brain TMS in Vancouver Canada - anybody have any suggestions about who to apply to?

Deep brain TMS study with paired PET scans for individualized targeted treatment based on individual brain functioning reads before and after treatment of deep TMS in identified areas in the PET...

09 March 2020 6,612 3 View

Can a cell membrane be overloaded with too many particles attempting to enter?

When more than one biomolecules attempt to enter into a cell membrane at once (in a large quantity) does it impede the transfection process?

23 July 2019 4,149 2 View

[solved] Can you explain a +44 mass difference in peptide synthesis?

Dear experts, after peptide synthesis (standard Fmoc chemistry, no acetylation between the couplings), I have a significant peak next to my main product for analytical reverse phase HPLC (see...

20 December 2018 782 7 View

Can you help me finding a list of transparent polymers suitable for light microscopy including refractive index?

We develop medical devices for characterization of thin tissues which should be produced from plastic/polymers (wall thickness

29 September 2018 5,445 8 View

Dear, could anyone point me to a good multivariate statistical analysis book?

I want to improve my knowledge in this area. I would like indications of good books that you have studied.

22 July 2018 4,867 10 View

What do you think of the Comet Assay?

Dear Investigator, The comet assay has been in existence for many years, but the general protocol largely remains unchanged, despite it requiring numerous, time-consuming steps. We in the...

12 October 2017 911 3 View

How to stop DAB precipitation during IHC

22 June 2017 2,939 3 View

How to determine oxygen amount / composition of a gas sample?

Hi, we recently had problems with some syntheses where we use nitrogen flow to remove a byproduct (tBu-SH) from a reduction reaction from a reaction mixture. The educt is a StBu-protected peptide...

17 January 2016 2,441 4 View

Which strategies exist to obtain high molecular weight polyesters?

Which strategies are known in literature to obtain high molecular weight polyesters? I am trying at the moment to react a semialiphatic alcohol at high temperatures with a aliphatic acid, using a...

13 January 2016 5,659 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View