Hello,
I am curious, do someone has the experiences with the the bacterial colonies aggregation (on the pictures), especially of B. subitlis, nearly behind or on the edge of inhibition (or rather no-growing) zone within Kirby-Bauer disc diffusion tests? And can I consider this phenomenon as the proof of the antibacterial activity of extracts 3, 4 and 5 or it has some other explanation, e.g. just some weak intolerance/sensitivity of bacterial strain for the metabolites followed by the transfer of highly mobile cells of B. subtilis to "non-toxic" zone? Thank you very much for each prospective reaction.
Hint for the pic.:
bacterial lawn - Bacillus subtilis culture inoculated from the liquid media Tryptic Soy Broth containing endospores of B. subtilis
samples on discs - 1,3,4,5,6 - different extracts of secondary metabolites from Streptomyces (after Ethyl acetate extraction, dissolved in methanol), 2 - Ethyl acetate control
Hi František Lorenc
The bacterium Bacillus subtilis , is an air contaminant and observed to be resistant towards prescribed antibiotics. The accumulation of bacteria near the edges could have been due to growth of persisters (Ref: Behera and Pattnaik, 2019, Biocatalysis and Agricultural Biotechnology, Elsevier).
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