Make different concentration of antibiotic-agar media and put them in microtitre plate's wells. Make a known concentration of bacteria by standard serial dilution plating method. Then inoculate the wells with unit amount of bacterial suspension (say, 10 microliter). Next day, just observe the growth in plate. You'll be able to see the exact point where the antimicrobial agents has stopped the growth of bacteria without using a microplate reader. This will be a preliminary result, but it'll help you for sure.