The NPs has to be dried and powdered or shall be left in suspension form for checking antimicrobial activity...
A simple method is the following:
Take a plate (Petri Dish) and add some gelatin.
Then bring your particles on a separated place on top of the gelatin. At a certain temperature, microorganisms will grow shortly. Take photographs and visualize the influence of your material.
good luck
By using 96- plates, simply adjust the bacterial growth at OD595 = 0.001 , add 100microliter from the bacterial broth in each well , then add 10 microliters from the nanoparticles, after 24h incubation , measure the whole plate OD using 96plate reader .
Also using the same method but u shape 96 plate for antibiofilm determination, and the time killing curve is very important as well, please find detailed methods in
Antibacterial activity of silver and zinc nanoparticles against vibrio cholerae and enterotoxogenic Escherichia coli, international journal of medical microbiology, salem et al 2015
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