How xanthine oxidase inhibitor might influence the renin: angiotensin system?

More Fariha Elwafi Elsakloul's questions See All

What could cause disturbed peaks in LCMS?

I am doing LC-MS (LC-QToF) of a phosphonate compound (nitrilotris methylene phosphonic acid), for which I add derivatization agent trimethylsilyl diazomethane to each sample and wait 2hours before...

04 March 2024 1,480 2 View

How can we improve HNSCC cell attachment and growth in primary cell line establishment?

We are attempting to establish primary cell lines from patient tumor samples of head and neck squamous cell carcinoma (HNSCC). Following transport to LUMS within 30-45 minutes of surgery, the...

01 January 2024 9,097 0 View

Has anyone else received this email?

Dear Prof. I hope this letter finds you well. It is my honor to extend to you an invitation to speak at our upcoming scholar's conference for researchers and students across Asia. Your...

16 December 2023 5,127 3 View

Has anyone else received this email? Is this the scam?

[email protected] Dear Prof. Fariha Kanwal I hope this letter finds you well. It is my honor to extend to you an invitation to speak at our upcoming scholar's conference for researchers and...

16 December 2023 4,906 66 View

How can i access code for "Multimodal Frame-Scoring Transformer for Video Summarization" research?

I am writing to express my admiration for the research work, particularly the paper titled "Multimodal Frame-Scoring Transformer for Video Summarization." The contributions to the field have been...

19 September 2023 1,173 0 View

Hi, can someone please enlighten me on why we use IL-12 and IL-18 stimulation on human NK cells for checking IFN-gamma production?

I want to check the cytotoxicity by measuring IFN-gamma production of human primary NK cells after co-incubation with a fungal pathogen.

27 June 2023 9,092 2 View

Hi, does anyone have experiences with the transfection of Primary Nk cells?

I want to do it but I'm a bit concerned about the viability at the end.

12 February 2023 7,114 2 View

Why my n-butanol fraction has dissolved all of my water fraction?

In series fraction, I’m on butanol solvent, when I added it to my water+extract base, on First addition it separated water clearly, on second addition of butanol it dissolved half of my water...

08 April 2021 8,142 10 View

Is International Journal of Pharmaceutical Research and Allied Sciences reliable? What is the impact factor of the journal?

Is International Journal of Pharmaceutical Research and Allied Sciences reliable and considerable for original article publication? what is its impact factor and h-index?

28 March 2021 9,473 1 View

How to make fractions of chloroform extract of plant, using seperating funnel?

I want to to do sequential fractionation of my plant’s chloroform extract using separating funnel. In which solvent should I dissolve my extract as my extract is immiscible in water.

28 February 2021 8,924 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

My article: The Extended Pedersen hypothesis can you correct the the name of first Author?

In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...

11 July 2024 1,801 2 View