How would I get Human Muscle sample cells and how we would store it before the extraction from mtDNA and Nuclear DNA from the sample, mean the protocols and all that?
Hi there,
check the link below:
http://link.springer.com/protocol/10.1385%2F0-89603-335-X%3A307
You extract mtDNA the same way as nuclear DNA. Are you talking about deriving your own cells from patients or asking where you can obtain them from?
your question is not very clear. Are you just interested in DNA isolation from muscle cells only or you want to extract both mtDNA and nuclear DNA from mammalian cells. The good and easiest would be to collect blood or saliva sample and you can simultaneously extract both mtDNA and nuclear DNA by using any method of DNA extraction. If you are just looking for muscle tissues as a source of both DNA, you need to get support of any clinician expert in tissue biopsies.
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