Can somebody tell me the best method for measuring cytotxicity for water preserved in plastic bottles?
Dear Manar,
Fauris et al. (1986) evaluated the materials in contact with water using the cell adhesion assay and RNA synthesis test on human HeLa S3 cells. Eight materials were tested:
1 cation resin, untreated and treated, one anionic resin untreated and treated, one epoxide resin, one polyvinyl chloride coating, one bitumen and one Teflon. A witness with ultra-pure water was used as reference. Contact conditions approximate the conditions of use of materials (3, 30 or 60 cm² / L). Three tests were carried out in (1) of the ultra-pure water for 24 hours, (2) ultra-pure chlorinated water for 24 hours and (3) of ultrapure water for 48 h.
In the first part, the authors studied the influence of temperature, the incubation period of the adhesion test and its reproducibility.
This study showed that all materials are cytotoxic (kinetic anchor and DNA synthesis) and there is a good correlation between the responses of the two tests. The adhesion test is slightly less sensitive but has the advantage of being much easier to implement.
Ref. Fauris C., Danglot C., H. Lundstrom, Vilagines R. (1986). Measuring induced toxicity materials in contact with drinking water.
French Journal of Hydrology 17 (2) 131-142.
With my best regards
Prof. Bachir ACHOUR
Put sterilised water on growing tissue culture repeatedly and see cytopathic affects like dead cells, less growth etc.
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