Can any one share with me the solubility data of 2-keto-l-gluconic acid in methanol ?

I want the solubility data of 2-keto-l-gluconic acid in methanol. Can any one share it with me?

31 December 2020 9,210 1 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Is it possible to perform cardiac puncture followed by PBS perfusion with a pump?

Hello all, I will be perfusing mice with PBS for brain harvest and a colleague would like to collect the blood via cardiac puncture (these are 18m old mice so would be nice to maximize use). Has...

15 February 2021 5,312 2 View

How to properly reach normality in residuals with lmer?

Hello, I have a large non-balanced panel dataset regarding a company dealing with private lessons for students. My goal is to fit a lmer model which predicts the number of monthly hours taken by a...

08 February 2021 2,539 7 View

CAS solution whit PIPES ?

To prepare CAS solution for siderophores assay, the protocol use anhydrous piperazine. Can I make this solution using PIPES ? And how? Thanks The protocol i'm following is:...

08 November 2020 2,751 2 View

Do you think that covid 19 virus separates through the air and make infections?

Do you think that covid 19 virus separates through the air and make infections?

14 September 2020 7,956 7 View

Any suggestions for a simple method to reduce the viscosity of bacterial growth media?

Hi, I am working with a bacteria from the genus Paenibacillus. I am having trouble separating it from the media (PDB), and difficulties in extraction of secondary metabolites from the media,...

04 August 2020 9,974 3 View

Can anyone suggest procedure to analyse solid state CD and UV-Vis spectra for chiral polymers?

To check CD and UV-Vis spectra for porous polymers.

28 May 2020 7,359 8 View

How can I improve my protein purification with Qiagen protocol-Ni-NTA column?

Hi, I purified a protein (about 28KD) via the following steps: 1- Firstly, I used buffer A native(Qiagen)-NaH2PO4,NACL,Immidazol,ph8 2-then after centrifuging and separating my pellet , I added...

13 May 2020 1,673 6 View

How to separate phospholipids from betaine lipids using silica chromatography?

I tried separating betaine lipid from phospholipid and galactolipid from my 2D TLC plate but to no avail. Betaine lipid was found to overlap with some galactolipids under the condition of:- 1D:...

25 March 2020 641 3 View

Do you think blood or bone marrow should be diluted before separating Buffy coat by LymphoSep?

First, do you think it is necessary to dilute blood or bone marrow samples before adding it on lymphoSep to separate the Buffy coat? OR simply add it on lymphoSep and then centrifuged. Second,...

04 March 2020 3,041 1 View