How we can selectively do the sulponation of the primary alcohol in presence of secondary alcohols using tosyl chloride?
Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .
04 February 2021 5,614 3 View
To who this may concern , I am trying to stain cross-section of a nerve with toluidine blue for morphometric analysis of the section. I am trying to identify the fibre diameters and general...
01 March 2020 3,986 8 View
Hi all, I am trying to immobilize Antibody by DTSSP on a gold substrate, The DTSSP has a center disulfide. Most of the research articles have not reported the reducing of DTSSP...
26 November 2019 8,742 1 View
Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...
20 December 2018 8,632 2 View
Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...
14 June 2018 7,144 4 View
Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...
11 April 2018 9,328 3 View
Every time in the NVT equilibration of the protein in the MD simulation using Gromacs, we are getting a termination on the run with an error stating segmentation fault (core dumped). The nvt.mdp...
06 February 2018 7,479 3 View
Hi, I have been working on Bacillus thuringiensis, when i amplify the Cry1 gene using genomic DNA i usually get a positive result. i need to know whether these Cry gene's are Genomic or...
25 October 2017 4,297 4 View
Hi, How to calculate 3 components of j integral using domain integral method.
14 May 2017 280 3 View
Hi, Is it possible to calculate displacement gradient matrix from strain tensor and gauss point co- ordinates
10 April 2017 6,741 3 View
there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...
27 February 2021 2,609 3 View
I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption
08 February 2021 7,493 3 View
The usual way of properly removing and collecting carbon nanotubes (CNT) is to sonicate the stainless steel plates coated with CNT under isopropyl alcohol (IPA) solution, remove the plates after...
28 January 2021 9,455 1 View
I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture. I got poked after sprayed them with 70 % alcohol and dried them right away. Is...
07 January 2021 9,412 2 View
I'm not sure the steps to treat wastewater containing mixture of alcohol solvent, oils and glycerol. do we neet to separate before further treatment.
27 December 2020 6,058 6 View
Hello everyone, I want to make some nanoparticles by free-radical copolymerization of some kind of monomers in a Miniemulsion state! However, my monomers are sensitive to hydrolysis by water and I...
22 December 2020 9,648 1 View
In discussion with some farmers and colleagues, I came to know that poultry birds are fed alcohols to enhance their production/performance and treat the respiratory infecions. Is there any...
13 December 2020 4,700 17 View
I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...
08 December 2020 4,643 2 View
In the preparation of a Lactophenol Cotton Blue, can phenol be substituted by benzul alcohol? (Question for some students in a laboratory lacking some reagents.)
03 December 2020 8,621 1 View
I am working on PANi/PVA self-standing films. For that I have dispersed PANi source i.e. emeraldine salt dispersed in NMP and ultrasonicated for few hours and the centrifuged to settle down the...
11 November 2020 831 3 View