If any one have simple method like HPLC, Spectrophotometry or other to determine the Ang-II level is tissues, please share with me.
For what reason do you want to measure in the eye?
I do not know any particular reason for measuring in an eye.
For general analysis you could try an ELISA method like http://www.enzolifesciences.com/ADI-900-204/angiotensin-ii-elisa-kit/
Hi Rajesh Choudhary,
If you only are interested in this protein and you have tissue-samples, I would use western-blotting or ELISA with appropriate controls. Which one is best, depends on your research question.
Good luck with your project.
Best regards,
Tue
Thanks everyone to your valuable suggestions.
Actually I have no idea about to mesearment but we think about ELISA method for detection.
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