How we can design a best primer for polymorphism investigation?

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Why different HCV patients with same genotype respond differently to interferon and Ribaviron combination?

can any explain some genetic differences responsible for EVR,RVR,SVR and NVR

02 March 2015 7,069 9 View

What is minimum requirement of Oxygen pressure for human breathing?

what is the minimum amount of oxygen in Torr for survival

10 November 2014 4,867 2 View

Can we use DNA extracted from serum for Polymorphism analysis?

I have serum samples and want to know if I can use that serum to get DNA for Polymorphism analysis

09 October 2014 1,869 3 View

Can we extract human DNA from Plasma ?

I have plasma samples and want to extract human DNA ,is to possible to extract the DNA from plasma ?

09 October 2014 9,362 20 View

What is the range for the forward primer and reverse primer and how can we identify the best primer?

Using a primer blast at NCBI.

07 August 2014 2,470 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to Input and Analyze Results for an Asymmetrical Factorial Design in STATISTICA 13.3?

In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...

29 July 2024 4,155 2 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Ahmed Mansour Alzohairy

This depends on how you design your experiment and what is the Molecular markers you are using. You can use random markers such as (RAPD, ISSR, IRAP...) or specific markers such as (SSR, ...). I hope this answer may help you

Data Comparative Assessment of Genetic Diversity in Tomato Cultiv...

Muhammad Sarfaraz Iqbal

Thanks sir I got my point from your answer

I have selected RFLP method for my project