How we can cultivate and produce high yield of Bacillus and its spore subtilis (Protocol) for livestock? If you have an idea let me know ?

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Jai Ghosh

I would recommend you a very simple way to get the answer to your question. Put this on Google search. You will get a large number of protocols. Now you select which one is suitable for you.

Khaled Khleifat

A 3-fold increase compared to that of previously highest rate made by Monteiro et al. (2005) for Bacillus subtilis spore production

Monteiro, S. M., Clemente, J. J., Henriques, A. O., Gomes, R. J., Carrondo, M. J., & Cunha, A. E. (2005). A Procedure for High‐Yield Spore Production by Bacillus s ubtilis. Biotechnology progress, 21(4), 1026-1031.