How we can add a mid-point root in an unrooted tree?

More Dr Varsha Rao's questions See All

Can anybody suggest to me some bioinformatics journals that don't charge for publishing?

11 December 2013 2,985 22 View

How to get promoter seuqence of a gene?

I want to search the promoter sequence of a gene. Seeking an advice on where to look for it.

09 October 2013 563 18 View

Can someone advise on the cloning of a gene?

While cloning I am only getting self ligated vector not any clone. I had digested Genomic DNA of my strain with Eco R1 as well as vector (PGEX). After ligation at 4C, i transformed the ligated...

08 September 2013 6,306 8 View

Why is there a difference in the production of enzymes in bacteria while sharing the same habitat and environmental conditions?

There are so many enzymes which are produced by some bacteria while others do not produce the same enzymes. How do we study the different mechanisms behind this?

08 September 2013 5,357 17 View

I have an question regarding plasmid (PET28a) DNA digestion?

I had found five bands after isolating PET28a vector by using a kit. I am using an EcoR1 enzyme for digesting PET28a vector but it is not digesting this vector. But along with this I had used...

07 August 2013 9,801 12 View

What is reason of not getting any band in SDS PAGE while protein activity was observed after performing protein assay?

I am working on an extracellular protein. After taking supernatant I performed protein assay and good activity of protein was observed but no band was observed when loaded on SDS PAGE.

07 August 2013 7,357 23 View

Problem in molecular visualization?

I performed docking of two molecules (one protein molecule and other one is its inhibitor) by using Hex software and saved the docked molecules. But when i am analyzing the same file in pymol and...

04 May 2013 1,544 3 View

How to interpret a phylogenetic rooted tree constructed by using Phylip software?

I have constructed a rooted tree by using the proml program of Phylip software. I have to find which organism is the primitive one but I am not able to calculate the distances of different nodes...

02 March 2013 1,228 12 View

Hi, can anybody tell me how to find out conserved domains in an protein?

I have to compare a single protein in different organisms.For that i want to know the conserved domains in the protein in the selected organisms.I was trying this by using KEGG database but iam...

10 November 2011 6,793 16 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity?

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...

03 August 2024 5,358 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Mikkel Schubert

Assuming that I understand your question correctly, then you could also use FigTree:

http://tree.bio.ed.ac.uk/software/figtree/

Just open your tree, and press Ctrl-M, or go to menu "Tree" -> "Midpoint Root".

Saurabh Gayali

I guess this page has info on retree command in phylip.

http://www0.nih.go.jp/~jun/research/phylip/retree.html