Dr Varsha Rao

10 Questions 22 Answers 0 Followers

Questions related from Dr Varsha Rao

Can anybody suggest to me some bioinformatics journals that don't charge for publishing?

12 December 2013 2,923 22 View

How to get promoter seuqence of a gene?

I want to search the promoter sequence of a gene. Seeking an advice on where to look for it.

10 October 2013 512 18 View

How we can add a mid-point root in an unrooted tree?

I am using Phylip for constructing the phylogenetic tree. I had used proml program and now I want to add an outgroup in the tree. Is this possible by phylip software or any tool?

10 October 2013 8,101 2 View

Can someone advise on the cloning of a gene?

While cloning I am only getting self ligated vector not any clone. I had digested Genomic DNA of my strain with Eco R1 as well as vector (PGEX). After ligation at 4C, i transformed the ligated...

09 September 2013 6,250 8 View

Why is there a difference in the production of enzymes in bacteria while sharing the same habitat and environmental conditions?

There are so many enzymes which are produced by some bacteria while others do not produce the same enzymes. How do we study the different mechanisms behind this?

09 September 2013 5,283 17 View

I have an question regarding plasmid (PET28a) DNA digestion?

I had found five bands after isolating PET28a vector by using a kit. I am using an EcoR1 enzyme for digesting PET28a vector but it is not digesting this vector. But along with this I had used...

08 August 2013 9,739 12 View

What is reason of not getting any band in SDS PAGE while protein activity was observed after performing protein assay?

I am working on an extracellular protein. After taking supernatant I performed protein assay and good activity of protein was observed but no band was observed when loaded on SDS PAGE.

08 August 2013 7,282 23 View

Problem in molecular visualization?

I performed docking of two molecules (one protein molecule and other one is its inhibitor) by using Hex software and saved the docked molecules. But when i am analyzing the same file in pymol and...

05 May 2013 1,498 3 View

How to interpret a phylogenetic rooted tree constructed by using Phylip software?

I have constructed a rooted tree by using the proml program of Phylip software. I have to find which organism is the primitive one but I am not able to calculate the distances of different nodes...

03 March 2013 1,175 12 View

Hi, can anybody tell me how to find out conserved domains in an protein?

I have to compare a single protein in different organisms.For that i want to know the conserved domains in the protein in the selected organisms.I was trying this by using KEGG database but iam...

11 November 2011 6,757 16 View