Is it possible to conduct a population genetic study using microsatellites in a lab where only normal gradient PCR and desalted primers are available?

Dear Experts, I have some experience in population genetics studies in plant systems using various chloroplast intergenic spacers (sequence data). Now, I would like to analyse a very restricted...

06 July 2019 4,749 4 View

How can I get 19 bioclimatic layers of CCSM4 rcp26?

I am trying to project the present climatic condition to 4 carbon emission scenario listed as CCSM4 rcp2.6. rcp45,rcp60,rcp85. But the layers are geotiff format, which is not recognised by...

11 December 2017 9,674 3 View

How can we infer ancestral relationships from an unrooted haplotype network?

It is common knowledge that internal nodes are ancestral and external nodes are of recent origin in a haplotype network. But most of the median joining haplotype networks published in articles...

11 December 2017 4,096 3 View

How to project present climatic conditions to LGM?

I am planning to perform SDM of an endangered tree in western ghats mountain range of India. When I do the projection of present conditions to the LGM, the distribution area overlaps with the sea....

11 December 2017 1,426 5 View

How to design test samples for maxent?

The total number of occurrence records is 23 (23 geographical coordinates) and if I want to make a test sample to run in maxent, how many points can be taken.

11 December 2017 9,620 6 View

How to insert traits and coordinates in PopART?

I would like to use PopART software for creating haplotype network. When I tried to upload the alignment of 122 sequences by inserting blocks for traits and coordinates, the software gets stuck by...

08 September 2017 8,520 10 View

How can we measure energy value of food?

31 December 2016 6,085 6 View

What is the best statistic to claim or not to claim 'significant difference between two data sets?

11 December 2016 3,451 9 View

Which instrument can be used for measuring frequency in Hertz?

I need to measure the frequency of various sound waves. May I know what is the best method/instrument for it.

11 December 2016 8,095 16 View

How to install Maps in Garmin Montana GPS650?

I am working on survey and collection of endangered medicinal plants of Western Ghats of India. I am using Garmin Montana GPS 650 for identifying geographical co-ordinates. But I am facing issues...

09 October 2016 9,472 4 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View