Is it possible to conduct a population genetic study using microsatellites in a lab where only normal gradient PCR and desalted primers are available?

Dear Experts, I have some experience in population genetics studies in plant systems using various chloroplast intergenic spacers (sequence data). Now, I would like to analyse a very restricted...

06 July 2019 4,749 4 View

How can I get 19 bioclimatic layers of CCSM4 rcp26?

I am trying to project the present climatic condition to 4 carbon emission scenario listed as CCSM4 rcp2.6. rcp45,rcp60,rcp85. But the layers are geotiff format, which is not recognised by...

11 December 2017 9,674 3 View

How can we infer ancestral relationships from an unrooted haplotype network?

It is common knowledge that internal nodes are ancestral and external nodes are of recent origin in a haplotype network. But most of the median joining haplotype networks published in articles...

11 December 2017 4,096 3 View

How to project present climatic conditions to LGM?

I am planning to perform SDM of an endangered tree in western ghats mountain range of India. When I do the projection of present conditions to the LGM, the distribution area overlaps with the sea....

11 December 2017 1,426 5 View

How to design test samples for maxent?

The total number of occurrence records is 23 (23 geographical coordinates) and if I want to make a test sample to run in maxent, how many points can be taken.

11 December 2017 9,620 6 View

How was Western Ghats of India formed?

I have tried to write an outline of the step-by-step processes that led to the formation of western ghats of India. Kindly go through the table and let me know where I have gone wrong!!!! Table...

10 November 2017 6,791 0 View

How to insert traits and coordinates in PopART?

I would like to use PopART software for creating haplotype network. When I tried to upload the alignment of 122 sequences by inserting blocks for traits and coordinates, the software gets stuck by...

08 September 2017 8,520 10 View

How can we measure energy value of food?

31 December 2016 6,085 6 View

What is the best statistic to claim or not to claim 'significant difference between two data sets?

11 December 2016 3,451 9 View

Which instrument can be used for measuring frequency in Hertz?

I need to measure the frequency of various sound waves. May I know what is the best method/instrument for it.

11 December 2016 8,095 16 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Fractional impervious surface (FIS) map ?

Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance

24 February 2021 8,455 2 View